음식물쓰레기 분해능을 갖는 효소활성 균주의 분리 및 동정

정한샘1, 채종찬2, 이승제1,*
Hansaem Jeong1, Jong-Chan Chae2, Seung-Je Lee1,*
Author Information & Copyright
1Jeonbuk Institute for Food-Bioindustry, Jeonju 54810, Korea
2Division of Biotechnology, Chonbuk National University, Iksan 54596, Korea
*Corresponding Author :

ⓒ Copyright 2017, Institute of Agricultural Science & Technology, Chonnam National University. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published Online: Dec 31, 2016


누룩과 음식물쓰레기로부터 내염성과 내열성을 갖는 균주를 분리한 후 탄수화물, 단백질, 지방 및 섬유소를 분해할 수 있는 효소활성 균주를 분리하고 동정하였다. 분리된 누룩 균주 74종으로부터 23종의 효소활성을 갖는 균주를 분리하였으며, 음식물쓰레기로부터는 11종을 분리하였다. 이 중에 서 내염성과 내열성을 가지면서 효소활성을 2가지 이상 갖는 균주 8종을 최종적으로 분리하여 분자생물학적 동정을 진행한 결과, 누룩으로부터는 Bacillus amyloliquefaciens, Acinetobactor baumannii, Enterobacter hormaechei, Saccharomycopsis fibuligera로 4종의 균주가 동정되었으며 음식물쓰레기로부터는 Raoultella ornithinolytics, Serratia marcescens로 2종의 균주가 동정되었다. 최종적으로 동정된 6종의 혼합균주를 분쇄된 음식물쓰레기에 24시간 분해시킨 결과, 음식물쓰레기의 감소 비율이 생중량 기준으로 52.7%, 그리고 건중량 기준으로 85.6%로 나타났다.


Food waste composed of 30-60% starch, 10-40% lipids and 5-10% proteins(w/w). The increase of food waste is one of the big problem which caused world economic loss and environmental damage. In this study, for the purpose of decomposing food waste, the strain was screened from Korean traditional Nuruk and food waste. Twenty three strains were isolated from 74 isolates of Nuruk, and eleventh strains were isolated from food waste. Among them, 8 kinds of strains which have salt resistance and heat resistance and two or more enzymatic activities among the amylase, cellulase, lipase, protease were finally isolated and subjected to molecular biology identification, were identified such as Bacillus amyloliquefaciens, Acinetobactor baumannii, Enterobacter hormaechei, Saccharomycopsis fibuligera, Raoultella ornithinolytics, Serratia marcescens, Raoultella ornithinolytica. Six species of mixed strains were inoculated into food waste, and the result after 24 hours decomposition, the reduction of food waste was 52.7% on as biomass basis.