Table 2. Silymarin contents and alcohol hydrolyzing effects of domestic thistles

Variety	Alcohol detoxification (mU/mL)	Silymarin contents
Variety	Alcohol detoxification (mU/mL)	Silybin (mg/g DW)	Isosilybin (mg/g DW)	Total (mg/g DW)
C. japonicum nakaisanum	6.40^c	2.87^c	0.51^d	3.38^d
C. japonicum spinosissimum	5.32^d	2.62^cd	1.75^c	4.37^c
C. crispus	2.13^e	2.17^d	1.58^c	3.75^d
C. japonicum	5.47^d	1.85^b	0.34^b	2.19^e
C. pendulum	15.25^a	34.54^a	26.71^a	61.24^a
C. setidens	8.57^b	5.82^b	16.66^b	22.48^b

All domestic thistles was extracted 80% ethanol for 3h at 60C using Soxhlet method and thistles extracts at 10μg/mL concentration was applied to detect its alcohol detoxification using alcohol dehydrogenase activity assay kit.